Question?
Answer: The iconic feature of fluorescence quantitative PCR detection is the PCR amplification curve. Whether the result is valid can be judged based on whether the linear change of the amplification curve conforms to the standard amplification linear change trend. (Note: The standard amplification exhibits an S-shaped smooth curve trend, with a certain increase in fluorescence signal. The original curve is
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Frequently Asked Questions
ACQUGEN Technology combines Molecular and Serology Assays on a single platform, offering genotyped results with unmatched sensitivity. It allows for multiplexing, enabling multiple patients and targets to be tested simultaneously, reducing costs and improving efficiency.
NexxGen Veterinary specializes in innovative molecular diagnostic technologies that enhance patient care and reduce laboratory costs. Our ACQUGEN Assays and Automated Target Sequencer revolutionize diagnostics with target-specific solutions.
Our team consists of experienced Laboratory Executives and Molecular Scientists with over 30 years of expertise in developing laboratory diagnostics, ensuring high-quality and innovative solutions for the veterinary industry.
The NGV-8 Plus is a portable, real-time PCR instrument that uses PCR-TaqMan technology for high sensitivity and specificity. It’s ideal for early diagnosis and targeted treatment of infectious diseases in pets, offering reliable and precise results.
The NGV-NP2 offers fast and efficient nucleic acid extraction in just 8 minutes. Its user-friendly design requires no professional staff, and the built-in ultraviolet sterilization prevents cross-contamination, making it perfect for veterinary settings.
Clean and maintain the PCR machine: Use 75% alcohol or wet wipes to first clean the external surfaces, removing dust and stains. Then, dry the surfaces with a dry tissue. (Note: When cleaning and wiping the hot cover and reaction hole, be sure not to drip liquid into the hole to avoid damaging the internal components.)
PCR machine: Observe if the running indicator lights are normal, check the running interface countdown, temperature display, Lid, and amplification curve for normal change. During normal operation, the amplification curve should start to appear around 40 minutes into the countdown. If not, there may be an issue with the operation. Please contact customer support promptly.
The Ct value represents the number of amplification cycles during the PCR process at which the fluorescence signal of the amplification product reaches the set fluorescence signal threshold. The Ct value is inversely proportional to the initial template concentration in the PCR reaction. The larger the Ct value, the lower the initial template concentration. In other words, a higher Ct
A negative nucleic acid test means that no nucleic acid of the relevant pathogen was detected in this experiment; a positive test means that the nucleic acid of the relevant pathogen was detected in the sample. When a nucleic acid test is positive, if the pet shows no symptoms, it is considered asymptomatic carrier. When the nucleic acid test is
If a pet shows clear symptoms, and all results from relevant testing projects are negative, considerations and analysis can be made from the following aspects: If all the above issues are ruled out, it may be that the pathogen is indeed not present in the sample or its concentration is below the detection limit. It’s possible that the detected pathogen
The situation where the two test results are inconsistent (both are the results of normal testing operations) can generally be divided into two situations: The first situation is different results from samples collected at different times: Because the sampling time and techniques may vary, and after undergoing two rounds of nucleic acid extraction and PCR amplification detection processes, especially for
As a primary principle, the pipette and its tips should not be mixed with others and should be used independently. Maintain proper usage of the pipette to avoid liquid backflow. It is recommended to clean the pipette with 75% alcohol before and after each experiment. Keep the pipette tips clean inside the box for proper use. It is recommended to
Wearing gloves or sealing waste is crucial for preventing nucleic acid contamination. It is essential to pay attention and avoid neglecting these measures. Notice: Wear disposable, powder-free latex/nitrile gloves when conducting experiments! Sampling gloves cannot be used directly for experimental operations; they must be replaced with new gloves. When opening the reagent tube cap each time, be sure to avoid
Clean and maintain the nucleic acid extractor: Use 75% alcohol or wet wipes to clean both the internal and external surfaces, removing dust and stains. Then, dry the surfaces with a dry tissue.
Nucleic acid automatic extractor: Observe if the indicator lights are in a normal state. The running process takes about 8 minutes, with the machine producing operational sounds every 30 seconds on average. After normal completion, the liquid inside the reagent cartridge should be clear without magnetic beads, and the magnetic sleeve should be in place with magnetic beads at the